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Bio-Rad rt pcr kit
Rt Pcr Kit, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 6773 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/iscript+rt+pcr+kit/IScript+C+DNA+Synthesis+Kit/pmc12906651-65-11-13
Average 99 stars, based on 6773 article reviews
rt pcr kit - by Bioz Stars, 2026-09
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Reverse Transcription Polymerase Chain Reaction:

Article Title: Role of mitochondrial dysfunction and biogenesis in fibromyalgia syndrome: Molecular mechanism in central nervous system.
Article Snippet: RNA was quantified with a spectrophotometer (NanoDrop Lite; Thermo Fisher Scientific, Wilmington, DE, USA) [60]. .. An iScript RT-PCR kit (Bio-Rad, Hercules, CA, USA) was used to synthesize first-strand cDNA according to the manufacturer's recommendations. ..

Article Title: Neuronutritional Enhancement Of Antioxidant Defense System through Nrf2/HO1/NQO1 axis in Fibromyalgia.
Article Snippet: Fibromyalgia (FM) is a disorder characterized by chronic widespread musculoskeletal pain, and it is often accompanied by fatigue, sleep disturbance, and cognitive dysfunction.. Although pathophysiology is multifactorial, increasing evidence highlights the pivotal role of oxidative stress and mitochondrial dysfunction in the development of this condition.. In particular, the Nrf2/HO-1/NQO1 antioxidant axis plays a crucial role in counteracting oxidative damage and maintaining cellular homeostasis.

Article Title: Freshwater pollution: cardiotoxicity effect of perfluorooctane sulfonic acid and neonicotinoid imidacloprid mixture.
Article Snippet: Perfluorooctanesulfonate (PFOS) is a widely used chemical that accumulates in living things and the environment, especially the aquatic, over time.. It is also known as a “forever chemical”.. Furthermore, different anthropogenic substances are rarely found individually in the environment.

Article Title: INDUCTION OF A NOTCH3 LEHMAN SYNDROME MUTATION IN OSTEOCYTES CAUSES OSTEOPENIA IN MALE C57BL/6J MICE
Article Snippet: Quantitative Reverse Transcription–Polymerase Chain Reaction (qRT-PCR) Total RNA was extracted with the micro RNeasy kit (Qiagen, Valencia, CA), in accordance with manufacturer’s instructions, as previously reported [ 7 , 10 , 41 , 42 ]. .. Equal amounts of RNA were reverse transcribed using the iScript RT-PCR kit (Bio-Rad) and amplified in the presence of specific primers (IDT) ( ) with SsoAdvanced Universal SYBR Green Supermix (Bio-Rad) at 60 °C for 35 cycles. .. Transcript copy number was estimated by comparison with a serial dilution of cDNA for Alpl (encoding for alkaline phosphatase from American Type Tissue Culture Collection (ATCC), Manassas, VA), Bglap (encoding for osteocalcin from J. Lian, University of Vermont), Sost (from Thermo Fisher Scientific, Waltham, MA), Tnfsf11 (encoding for RANKL from Source BioScience, Nottingham, UK), Tnfrsf11b (encoding for osteoprotegerin from ATCC), or Notch3 PEST (U. Lendahl, Addgene #47618, Watertown, MA) [ 43 ].

Article Title: Oxidative stress and mitochondrial dysfunction in brain of vinclozolin exposed animals.
Article Snippet: RNA was quantified with a spectrophotometer (NanoDrop Lite; Thermo Fisher Scientific) (Gugliandolo et al., 2020). .. An iScript RT-PCR kit (Bio-Rad, Hercules, CA, USA) was used to synthesize first-strand cDNA according to the manufacturer’s recommendations. .. A total of 1 μL of complete cDNA was employed for real-time PCR analysis utilizing the SYBR Green method on a Real-Time PCR System from Applied Biosystems (Marino et al., 2023).

Article Title: Impaired mitochondrial quality control in fibromyalgia: Mechanisms involved in skeletal muscle alteration.
Article Snippet: RNA was quantified with a spectrophotometer (NanoDrop Lite) [38]. .. An iScript RT-PCR kit (Bio-Rad, Hercules, CA, USA) was used to synthesize first-strand cDNA as described by the manufacturer’s recommendations. .. A total of 1 μL of complete cDNA was employed for real-time PCR analysis utilizing the SYBR Green method on a Real-Time PCR System from Applied Biosystems [39].

Article Title: NOTCH2 sensitizes the chondrocyte to the inflammatory response of tumor necrosis factor α.
Article Snippet: The integrity of the RNA was assessed in random samples by microfluidic electrophoresis on an Experion system (Bio-Rad), and RNA with a quality indicator number equal to or higher than 7.0 was used for subsequent analysis. .. Equal amounts of RNA were reverse-transcribed using the iScript RT-PCR kit (Bio-Rad) and amplified in the presence of specific primers (Table 2) (all from IDT) with the SsoAdvanced Universal SYBR Green Supermix (Bio-Rad) at 60 C for 40 cycles. .. Transcript copy number was estimated by comparison with a serial dilution of cDNA for Acan, Il6, Illb, Notch2, Col10a1, Sox9, and Rbpj (from Thermo Fisher Scientific), Hes1, Col2a1, and Rpl38 (American Type Culture Collection), Prg4 (Bioscience), Hey1 and Hey2 (T. Iso, Gunma University) and Heyl (D. Srivastava, Gladstone Institute of Cardiovascular Disease or Dharmacon, Horizon Discovery) (65, 66).

Reverse Transcription:

Article Title: INDUCTION OF A NOTCH3 LEHMAN SYNDROME MUTATION IN OSTEOCYTES CAUSES OSTEOPENIA IN MALE C57BL/6J MICE
Article Snippet: Quantitative Reverse Transcription–Polymerase Chain Reaction (qRT-PCR) Total RNA was extracted with the micro RNeasy kit (Qiagen, Valencia, CA), in accordance with manufacturer’s instructions, as previously reported [ 7 , 10 , 41 , 42 ]. .. Equal amounts of RNA were reverse transcribed using the iScript RT-PCR kit (Bio-Rad) and amplified in the presence of specific primers (IDT) ( ) with SsoAdvanced Universal SYBR Green Supermix (Bio-Rad) at 60 °C for 35 cycles. .. Transcript copy number was estimated by comparison with a serial dilution of cDNA for Alpl (encoding for alkaline phosphatase from American Type Tissue Culture Collection (ATCC), Manassas, VA), Bglap (encoding for osteocalcin from J. Lian, University of Vermont), Sost (from Thermo Fisher Scientific, Waltham, MA), Tnfsf11 (encoding for RANKL from Source BioScience, Nottingham, UK), Tnfrsf11b (encoding for osteoprotegerin from ATCC), or Notch3 PEST (U. Lendahl, Addgene #47618, Watertown, MA) [ 43 ].

Article Title: NOTCH2 sensitizes the chondrocyte to the inflammatory response of tumor necrosis factor α.
Article Snippet: The integrity of the RNA was assessed in random samples by microfluidic electrophoresis on an Experion system (Bio-Rad), and RNA with a quality indicator number equal to or higher than 7.0 was used for subsequent analysis. .. Equal amounts of RNA were reverse-transcribed using the iScript RT-PCR kit (Bio-Rad) and amplified in the presence of specific primers (Table 2) (all from IDT) with the SsoAdvanced Universal SYBR Green Supermix (Bio-Rad) at 60 C for 40 cycles. .. Transcript copy number was estimated by comparison with a serial dilution of cDNA for Acan, Il6, Illb, Notch2, Col10a1, Sox9, and Rbpj (from Thermo Fisher Scientific), Hes1, Col2a1, and Rpl38 (American Type Culture Collection), Prg4 (Bioscience), Hey1 and Hey2 (T. Iso, Gunma University) and Heyl (D. Srivastava, Gladstone Institute of Cardiovascular Disease or Dharmacon, Horizon Discovery) (65, 66).

Amplification:

Article Title: INDUCTION OF A NOTCH3 LEHMAN SYNDROME MUTATION IN OSTEOCYTES CAUSES OSTEOPENIA IN MALE C57BL/6J MICE
Article Snippet: Quantitative Reverse Transcription–Polymerase Chain Reaction (qRT-PCR) Total RNA was extracted with the micro RNeasy kit (Qiagen, Valencia, CA), in accordance with manufacturer’s instructions, as previously reported [ 7 , 10 , 41 , 42 ]. .. Equal amounts of RNA were reverse transcribed using the iScript RT-PCR kit (Bio-Rad) and amplified in the presence of specific primers (IDT) ( ) with SsoAdvanced Universal SYBR Green Supermix (Bio-Rad) at 60 °C for 35 cycles. .. Transcript copy number was estimated by comparison with a serial dilution of cDNA for Alpl (encoding for alkaline phosphatase from American Type Tissue Culture Collection (ATCC), Manassas, VA), Bglap (encoding for osteocalcin from J. Lian, University of Vermont), Sost (from Thermo Fisher Scientific, Waltham, MA), Tnfsf11 (encoding for RANKL from Source BioScience, Nottingham, UK), Tnfrsf11b (encoding for osteoprotegerin from ATCC), or Notch3 PEST (U. Lendahl, Addgene #47618, Watertown, MA) [ 43 ].

Article Title: NOTCH2 sensitizes the chondrocyte to the inflammatory response of tumor necrosis factor α.
Article Snippet: The integrity of the RNA was assessed in random samples by microfluidic electrophoresis on an Experion system (Bio-Rad), and RNA with a quality indicator number equal to or higher than 7.0 was used for subsequent analysis. .. Equal amounts of RNA were reverse-transcribed using the iScript RT-PCR kit (Bio-Rad) and amplified in the presence of specific primers (Table 2) (all from IDT) with the SsoAdvanced Universal SYBR Green Supermix (Bio-Rad) at 60 C for 40 cycles. .. Transcript copy number was estimated by comparison with a serial dilution of cDNA for Acan, Il6, Illb, Notch2, Col10a1, Sox9, and Rbpj (from Thermo Fisher Scientific), Hes1, Col2a1, and Rpl38 (American Type Culture Collection), Prg4 (Bioscience), Hey1 and Hey2 (T. Iso, Gunma University) and Heyl (D. Srivastava, Gladstone Institute of Cardiovascular Disease or Dharmacon, Horizon Discovery) (65, 66).

SYBR Green Assay:

Article Title: INDUCTION OF A NOTCH3 LEHMAN SYNDROME MUTATION IN OSTEOCYTES CAUSES OSTEOPENIA IN MALE C57BL/6J MICE
Article Snippet: Quantitative Reverse Transcription–Polymerase Chain Reaction (qRT-PCR) Total RNA was extracted with the micro RNeasy kit (Qiagen, Valencia, CA), in accordance with manufacturer’s instructions, as previously reported [ 7 , 10 , 41 , 42 ]. .. Equal amounts of RNA were reverse transcribed using the iScript RT-PCR kit (Bio-Rad) and amplified in the presence of specific primers (IDT) ( ) with SsoAdvanced Universal SYBR Green Supermix (Bio-Rad) at 60 °C for 35 cycles. .. Transcript copy number was estimated by comparison with a serial dilution of cDNA for Alpl (encoding for alkaline phosphatase from American Type Tissue Culture Collection (ATCC), Manassas, VA), Bglap (encoding for osteocalcin from J. Lian, University of Vermont), Sost (from Thermo Fisher Scientific, Waltham, MA), Tnfsf11 (encoding for RANKL from Source BioScience, Nottingham, UK), Tnfrsf11b (encoding for osteoprotegerin from ATCC), or Notch3 PEST (U. Lendahl, Addgene #47618, Watertown, MA) [ 43 ].

Article Title: NOTCH2 sensitizes the chondrocyte to the inflammatory response of tumor necrosis factor α.
Article Snippet: The integrity of the RNA was assessed in random samples by microfluidic electrophoresis on an Experion system (Bio-Rad), and RNA with a quality indicator number equal to or higher than 7.0 was used for subsequent analysis. .. Equal amounts of RNA were reverse-transcribed using the iScript RT-PCR kit (Bio-Rad) and amplified in the presence of specific primers (Table 2) (all from IDT) with the SsoAdvanced Universal SYBR Green Supermix (Bio-Rad) at 60 C for 40 cycles. .. Transcript copy number was estimated by comparison with a serial dilution of cDNA for Acan, Il6, Illb, Notch2, Col10a1, Sox9, and Rbpj (from Thermo Fisher Scientific), Hes1, Col2a1, and Rpl38 (American Type Culture Collection), Prg4 (Bioscience), Hey1 and Hey2 (T. Iso, Gunma University) and Heyl (D. Srivastava, Gladstone Institute of Cardiovascular Disease or Dharmacon, Horizon Discovery) (65, 66).



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